Fast Protein Liquid Chromatography (FPLC), a form of medium-pressure chromatography that uses a pump to control the speed at which the mobile phase passes through the stationary phase. UNICORN 7 WrkStn control software. The fast protein liquid chromatography system we are going to purchase has column switching valves that allow the user to switch between columns, sample pumps to apply the sample to a column, and buffer blending valves that can generate desired buffer gradients, enabling most user configuration and method creation via a dedicated software interface.